- Title
- Intracranial pressure elevation 24 h after ischemic stroke in aged rats is prevented by early, short hypothermia treatment
- Creator
- Murtha, Lucy A.; Beard, Daniel J.; Bourke, Julia T.; Pepperall, Debbie; McLeod, Damian D.; Spratt, Neil J.
- Relation
- NHMRC.1033461 and 1035465 http://purl.org/au-research/grants/nhmrc/1035465
- Relation
- Frontiers in Aging Neuroscience Vol. 8, no. 124
- Publisher Link
- http://dx.doi.org/10.3389/fnagi.2016.00124
- Publisher
- Frontiers Research Foundation
- Resource Type
- journal article
- Date
- 2016
- Description
- Stroke is predominantly a senescent disease, yet most preclinical studies investigate treatment in young animals. We recently demonstrated that short-duration hypothermia-treatment completely prevented the dramatic intracranial pressure (ICP) rise seen post-stroke in young rats. Here, our aim was to investigate whether a similar ICP rise occurs in aged rats and to determine whether short-duration hypothermia is an effective treatment in aged animals. Experimental middle cerebral artery occlusion (MCAo-3 h occlusion) was performed on male Wistar rats aged 19-20 months. At 1 h after stroke-onset, rats were randomized to 2.5 h hypothermia-treatment (32.5°C) or normothermia (37°C). ICP was monitored at baseline, for 3.5 h post-occlusion, and at 24 h post-stroke. Infarct and edema volumes were calculated from histology. Baseline pre-stroke ICP was 11.2 ± 3.3 mmHg across all animals. Twenty-four hours post-stroke, ICP was significantly higher in normothermic animals compared to hypothermia-treated animals (27.4 ± 18.2 mmHg vs. 8.0 ± 5.0 mmHg, p = 0.03). Infarct and edema volumes were not significantly different between groups. These data demonstrate ICP may also increase 24 h post-stroke in aged rats, and that short-duration hypothermia treatment has a profound and sustained preventative effect. These findings may have important implications for the use of hypothermia in clinical trials of aged stroke patients.
- Subject
- ischemic stroke; intracranial pressure; therapeutic hypothermia; aged rats; middle cerebral artery occlusion; cerebral edema
- Identifier
- http://hdl.handle.net/1959.13/1345636
- Identifier
- uon:29687
- Identifier
- ISSN:1663-4365
- Rights
- © 2016 Murtha, Beard, Bourke, Pepperall, McLeod and Spratt. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms
- Language
- eng
- Full Text
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